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American Society of Microbiology ABMM Sample Questions:

1. A previously healthy 28-year-old female develops a severe, watery diarrhea after attending a picnic where she consumed potato salad that had been left unrefrigerated for several hours. Stool culture reveals numerous Gram-positive bacilli. Further biochemical testing shows the organism is beta-hemolytic on blood agar and produces a heat-stable enterotoxin. The MOST likely causative agent is:

A) Staphylococcus aureus
B) Bacillus cereus
C) Clostridium difficile
D) Vibrio cholerae

2. A Klebsiella pneumoniae isolate from a bloodstream infection shows the following susceptibility profile:
Meropenem (MIC = 8 µg/mL, R), Imipenem (MIC = 16 µg/mL, R), Ertapenem (MIC = 4 µg/mL, R), Ceftazidime/avibactam (MIC = 1 µg/mL, S), Aztreonam (MIC = 32 µg/mL, R). Which resistance mechanism is most strongly suggested by this pattern?

A) Extended-Spectrum Beta-Lactamase (ESBL) production alone
B) Metallo-beta-lactamase (MBL) production
C) AmpC beta-lactamase hyperproduction coupled with porin loss
D) Klebsiella pneumoniae Carbapenemase (KPC) production

3. Microscopic examination of a trichrome-stained stool smear reveals amoebic trophozoites approximately 25
µm in diameter, containing ingested red blood cells. The nucleus, when visible, has fine, evenly distributed peripheral chromatin and a small, central karyosome. These features are diagnostic for:

A) Endolimax nana
B) Entamoeba histolytica
C) Iodamoeba bütschlii
D) Entamoeba coli

4. A patient with a history of neutropenia develops a severe sinus infection. Tissue biopsy reveals broad, ribbon- like, non-septate hyphae with right-angle branching. The MOST likely causative organism belongs to the order:

A) Onygenales
B) Mucorales
C) Aspergillales
D) Eurotiales

5. A diagnostic laboratory is validating a new multiplex PCR panel for respiratory pathogens. According to CLIA regulations, which of the following parameters must be established by the laboratory performing the validation for this qualitative nucleic acid amplification test?

A) Accuracy, Precision, Reportable Range, Analytical Sensitivity
B) Accuracy, Precision, Clinical Sensitivity, Clinical Specificity
C) Accuracy, Precision, Analytical Sensitivity, Analytical Specificity (including cross-reactivity)
D) Precision, Reportable Range, Analytical Specificity, Reference Interval

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